Activation of toll-like receptor 4 induces downregulation of α7 nicotinic acetylcholine receptor in microglia
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- Nakamura Yoki
- Dept. Pharmacol., Grad. Sch. Biomed. & Health Sci., Hiroshima Univ.
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- Kimura Sayuri
- Dept. Pharmacol., Grad. Sch. Biomed. & Health Sci., Hiroshima Univ.
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- Takemura Masatoshi
- Dept. Pharmacol., Grad. Sch. Biomed. & Health Sci., Hiroshima Univ.
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- Kodama Keitaro
- Dept. Pharmacol., Grad. Sch. Biomed. & Health Sci., Hiroshima Univ.
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- Iwamoto Momoka
- Dept. Pharmacol., Grad. Sch. Biomed. & Health Sci., Hiroshima Univ.
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- Nakashima Kazue
- Dept. Pharmacol., Grad. Sch. Biomed. & Health Sci., Hiroshima Univ.
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- Nakata Yoshihiro
- Dept. Pharmacol., Grad. Sch. Biomed. & Health Sci., Hiroshima Univ.
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- Morioka Norimitsu
- Dept. Pharmacol., Grad. Sch. Biomed. & Health Sci., Hiroshima Univ.
Bibliographic Information
- Other Title
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- Toll-like receptor 4 の活性化はミクログリアの α7 ニコチン受容体発現減少を引き起こす
Abstract
<p>Background: Inflammatory responses could be involved in induction of Alzheimer's disease (AD). Microglia are known to act as the main immunologic effector cell in the central nervous system, and contribute to the pathological states of AD. Activation of α7 nicotinic acetylcholine receptor (α7-nAChR) potentiated microglial phagocytosis of amyloid-β. By contrast, α7-nAChR was decreased along with the progression of AD in rodent models. The current study has investigated the mechanisms underlying downregulation of microglial α7-nAChR expression by activation of toll-like receptor 4 (TLR4).</p><p>Method: Mice microglial cell-line BV2 cells were used for investigation of downregulation of α7-nAChR expression. The mRNA expression levels of α7-nAChR was measured by the real-time PCR.</p><p>Results: Treatment of BV2 cells with lipopolysaccharide (LPS) significantly decreased the expression of α7-nAChR in dose- and time dependent manner. The LPS-induced downregulation of α7-nAChR mRNA was mediated by TLR4 and histone deacetylase (HDAC), but not DNA methyltransferase.</p><p>Conclusion: The current study demonstrated that the TLR4-HDAC pathway might contribute to the downregulation of α7-nAChR in microglia.</p>
Journal
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- Proceedings for Annual Meeting of The Japanese Pharmacological Society
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Proceedings for Annual Meeting of The Japanese Pharmacological Society 92 (0), 1-P-016-, 2019
Japanese Pharmacological Society
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Keywords
Details 詳細情報について
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- CRID
- 1390846609814406912
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- NII Article ID
- 130007812561
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- ISSN
- 24354953
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- Text Lang
- ja
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed