Effects of purine and pyrimidine nucleosides on hydrogen peroxide-induced thymidine incorporation

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  • Tanaka Koh-ichi
    Div. Pharmacol., Dept. of Pharm., Sch. Pharm., Hyogo Univ. Health Sci. Dept. Pharmacol., Hyogo Col. Med. Dept. Applied Pharmacol., Kagoshima Univ. Grad. Sch. Med. & Dent. Sci.
  • Tomita Kazuo
    Div. Pharmacol., Dept. of Pharm., Sch. Pharm., Hyogo Univ. Health Sci. Dept. Applied Pharmacol., Kagoshima Univ. Grad. Sch. Med. & Dent. Sci.
  • Kitanaka Nobue
    Dept. Pharmacol., Hyogo Col. Med.
  • Kitanaka Junichi
    Dept. Pharmacol., Hyogo Col. Med.
  • Sato Tomoaki
    Dept. Applied Pharmacol., Kagoshima Univ. Grad. Sch. Med. & Dent. Sci.
  • Takemura Motohiko
    Dept. Pharmacol., Hyogo Col. Med.
  • Nishiyama Nobuyoshi
    Div. Pharmacol., Dept. of Pharm., Sch. Pharm., Hyogo Univ. Health Sci.

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Other Title
  • プリン・ピリミジンヌクレオシドの過酸化水素によるチミジン取り込みへの効果

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<p>We have found that cultured differentiated astrocytes pretreated with N6, 2'-O-dibutyryladenosine 3',5'-cyclic monophosphate (DBcAMP), a permeable analogue of cAMP, incorporate thymidine, but not uridine, via specific nucleoside transporters, ENT2 and CNT3, into TCA insoluble fraction for repair on DNA injury in the presence of hydrogen peroxide (H2O2) at an early time, and these phenomena are specific in differentiated astrocytes, but not undifferentiated astrocytes and neurons. Purine and pyrimidine nucleosides are DNA and RNA precursors consisting of base and ribose.</p><p>We studied the influence of purine and pyrimidine nucleosides on H2O2-induced thymidine incorporation into cultured astrocytes. Adenosine and guanosine, RNA precursor purine nucleosides, decreased H2O2-induced thymidine incorporation. On the other hand, Deoxyadnosine and deoxyguanosine, DNA precursor purine nucleosides, increased it. Cytidine, RNA precursor pyrimidine nucleoside, did not influence it, but deoxycitidine, DNA precursor pyrimidine nucleoside, decreased it.</p><p>These results indicate that H2O2-induced thymidine incorporation into cultured astrocytes are increased synergistically by purine nucleosides, but not pyrimidine nucleosides.</p>

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