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- 田島 雅道
- 明海大・歯・歯科薬理
書誌事項
- タイトル別名
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- Bisphosphonates induce osteoblastic cell death through the inhibition of autophagic flux
抄録
<p>Extracting teeth of patients treated with bisphosphonates (BPs) occasionally induces the necrosis of jaw (BRONJ), but the cause of disease is still unclear. I found out that the intracellular BP of osteoblastic cells was gradually accumulated in lysosomes. In the present study, I investigated the mechanism of osteoblastic cell death induced by BPs. MC3T3-E1 cells were used as osteoblatic cells. The uptake of BP into cells was observed by fluorescent pamidronate. Apoptosis was evaluated by PSVue 480 and CellEvent caspase 3/7. Formation of autophagosome and autolysosome was observed using DAPGreen and DALGreen respectively. Autophagosome and autolysosome were frequently observed in the cytosol of normal MC3T3-E1 cells. On the other hands, autolysosomes of cells treated with BPs were gradually accumulated around nuclei. The mitochondria of BPs-treated cells were decreased in both response and quantity. Moreover, BPs shrank nuclei of cells. After a while phosphatidylserine (early apoptotic marker) exposed on cell membranes was detected, and finally the activation of caspase 3 was observed. Mitochondria in cells treated with BPs were not co-localized with autolysosomes. These results suggest that BPs may accumulate autolysosome around nuclei of osteoblastic cells, and induce apoptosis due to inhibiting autophagic flux.</p>
収録刊行物
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- 日本薬理学会年会要旨集
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日本薬理学会年会要旨集 92 (0), 1-P-120-, 2019
公益社団法人 日本薬理学会
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詳細情報 詳細情報について
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- CRID
- 1390846609814630784
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- NII論文ID
- 130007812742
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- ISSN
- 24354953
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可