Induction of cystic ovarian follicles (COFs) in cattle by using an intrafollicular injection of indomethacin
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- LAPP Rebecca
- Institute of Reproductive Biology, Leibniz Institute for Farm Animal Biology (FBN), D-18196 Dummerstorf, Germany
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- RÖTTGEN Volker
- Institute of Reproductive Biology, Leibniz Institute for Farm Animal Biology (FBN), D-18196 Dummerstorf, Germany Institute of Behavioural Physiology, Leibniz Institute for Farm Animal Biology (FBN), D-18196 Dummerstorf, Germany
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- VIERGUTZ Torsten
- Institute of Reproductive Biology, Leibniz Institute for Farm Animal Biology (FBN), D-18196 Dummerstorf, Germany
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- WEITZEL Joachim M.
- Institute of Reproductive Biology, Leibniz Institute for Farm Animal Biology (FBN), D-18196 Dummerstorf, Germany
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- VERNUNFT Andreas
- Institute of Reproductive Biology, Leibniz Institute for Farm Animal Biology (FBN), D-18196 Dummerstorf, Germany
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<p> The aim of this study was to establish a model to induce cystic ovarian follicles (COFs) in cattle using the cyclooxygenase inhibitor, indomethacin. Eighteen Holstein-Frisian cattle were synchronized with prostaglandin F2alpha (PGF2α) and gonadotropin-releasing hormone (GnRH). Ultrasound-guided transvaginal intrafollicular injections were performed in 23 preovulatory follicles with different concentrations of indomethacin 16 h after GnRH administration. An injection of 0.2 ml 35 µM indomethacin solution (resulting in a final concentration of 8 µg/ml in the follicular fluid) was the minimal dosage leading to COF formation. The induced COFs reached a maximum mean diameter of 36.9 ± 4.5 mm eleven days after injection. The estrous cycle was extended to 25–39 days. Luteinization was first observed 4 days after injection, accompanied by a slight increase in plasma progesterone concentration. The bioactivity of indomethacin was demonstrated by the decrease of prostaglandin E2 in the follicular fluid of three animals. The method presented here is minimally invasive and allows for the generation of defined COFs for further investigations.</p>
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 66 (2), 181-188, 2020
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390846609823902208
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- NII論文ID
- 130007830112
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- NII書誌ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 030371727
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- PubMed
- 31983719
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可