Practical Method to Identify Tree Peony Cultivars Using RAPD Markers

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  • Mochida Kohei
    United Graduate School of Agricultural Science, Tottori University Shimane Agricultural Technology Center
  • Kako Tetsuya
    United Graduate School of Agricultural Science, Tottori University Shimane Agricultural Technology Center
  • Sugiyama Mari
    Shimane Agricultural Technology Center
  • Nakatsuka Akira
    Faculty of Life and Environmental Science, Shimane University
  • Kobayashi Nobuo
    Faculty of Life and Environmental Science, Shimane University

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Other Title
  • ボタンにおけるRAPDマーカーを用いた実用的な品種識別法の確立
  • ボタン ニ オケル RAPD マーカー オ モチイタ ジツヨウテキ ナ ヒンシュ シキベツホウ ノ カクリツ

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Abstract

<p>To establish a practical method to identify tree peony cultivars, we attempted to develop a method to distinguish 353 tree peony cultivars cited in the “Botan meikan” directory using Random Amplified Polymorphic DNA (RAPD) markers. Using 29 primers, 48 RAPD markers that identify polymorphisms among peony cultivars were obtained. All of the cultivars, except eight bud mutation groups and two presumed synonymous cultivars, could be distinguished using the 48 RAPD markers. Parents, offspring, and sibling cultivars could be distinguished using these markers. On the comparison of RAPD band patterns between cultivar groups, specific bands of each cultivar group were obtained. To confirm the effectiveness of the developed markers, cultivar identification was performed using specimens in an exhibition garden. The cultivar names inferred from RAPD markers were matched with the labeled names in 91.4% of the tested individuals. Thus, the RAPD markers developed in this study are effective for the curation of tree peony cultivars.</p>

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