Novel physico-chemical regulation mechanism enables the production of 100 billion platelets

DOI Web Site PubMed
  • NAKAMURA Sou
    Department of Clinical Application, Center for iPS Cell Research and Application, Kyoto University
  • ETO Koji
    Department of Clinical Application, Center for iPS Cell Research and Application, Kyoto University Department of Regenerative Medicine, Chiba University Graduate School of Medicine

Bibliographic Information

Other Title
  • 新規物理化学制御機構が1,000億個の血小板作製を可能にする
  • 学会奨励賞受賞論文 新規物理化学制御機構が1,000億個の血小板作製を可能にする
  • ガッカイ ショウレイショウ ジュショウ ロンブン シンキ ブツリ カガク セイギョ キコウ ガ 1,000オクコ ノ ケッショウバン サクセイ オ カノウ ニ スル

Search this article

Abstract

<p>Since induced pluripotent stem (iPS) cell-derived blood products can be produced from any individual, they are expected to complement current transfusion products. However, a main problem is how to produce 10 U platelet preparations. Therefore, we established an immortalized megakaryocyte cell line (imMKCL) from iPS cells. We also found that turbulent flow was an essential physical factor for platelet generation in vivo. This knowledge enabled us to obtain 100 billion functional platelets from imMKCL using an 8 L bioreactor. We propose that the enhanced platelet production in the bioreactor occurs due to the turbulent flow that promoted the release of stress-induced cytokines.</p>

Journal

  • Rinsho Ketsueki

    Rinsho Ketsueki 61 (6), 628-633, 2020

    The Japanese Society of Hematology

Related Projects

See more

Keywords

Details 詳細情報について

Report a problem

Back to top