Simultaneous Determination of 7 Short-Chain Fatty Acids in Human Saliva by High-Sensitivity Gas Chromatography-Mass Spectrometry

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  • KAWASE Takahiro
    Kyoto Institute of Nutrition & Pathology
  • HATANAKA Kazu
    Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Department of Pathophysiology - Periodontal Science
  • KONO Mari
    Sysmex Corporation, Scietific affairs
  • SHIRAHASE Yasushi
    Sysmex Corporation, Scietific affairs
  • OCHIAI Kuniyasu
    Nihon University, School of Dentistry
  • TAKASHIBA Shogo
    Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Department of Pathophysiology - Periodontal Science
  • TSUKAHARA Takamitsu
    Kyoto Institute of Nutrition & Pathology

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Description

<p>Upon absorption in the intestine of the host animal, the main function of short-chain fatty acids (SCFAs), mainly acetate, propionate and n-butyrate, is as metabolic energy. SCFAs, n-butyrate in particular, can also be found in the mouth. An excess of oral SCFAs may cause not only periodontal diseases but also systemic abnormalities in humans. Previously, we reported a method for simultaneous detection by gas chromatography-mass spectrometry (GC-MS) of acetate, propionate and n-butyrate in serum, urine and saliva. In the present study we used a modified version of this method to detect not only acetate, propionate and n-butyrate, but also iso-butyrate, n-valerate, iso-valerate and caproate, because the latter are suggested to be associated with periodontal diseases. Detection ranges of SCFAs were as follows; 6.25-400 µmol/L (acetate), 0.781-100 µmol/L (propionate and n-butyrate), 0.391-50 µmol/L (iso-butyrate), 0.781-50 µmol/L (n-valerate and iso-valerate) and 1.56-50 µmol/L (caproate). Furthermore, we validated the modified detection method with triple freeze-thaw-cycle recovery tests and intra- and inter-day repeatability. Freezing and thawing did not influence the concentrations of SCFAs in saliva. Upon analysis of five clinical saliva samples, it was observed that, except for n-valerate, which was detected only in two samples, all SCFAs were detected in saliva samples. To conclude, we were able to use a modified method to analyze successfully by GC-MS the salivary concentrations of SCFAs. In addition, we simultaneously detected the salivary concentrations of iso-butyrate, iso-valerate, nvalerate and caproate. This improved method was proved to be reliable to measure the concentrations of SCFAs in saliva.</p>

Journal

  • CHROMATOGRAPHY

    CHROMATOGRAPHY 41 (2), 63-71, 2020-06-20

    The Society for Chromatographic Sciences

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