Preparation of Si-containing calcium phosphate thin film and evaluation of cell adhesion


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  • Si 含有リン酸カルシウム薄膜の作製と細胞接着性評価


Ti implants have been used primarily for current dental implants.However the peri-implant inflammation is a serious ploblem.In order to improve the adhesion between the abutment and the epithelium,Si-containing calcium phosphate thin film containing Si-Ca-P was coated on Ti using sputtering method from a mixture of hydroxyapatite and SiO2 as a target.Then,cell adhesion on the thin film was evaluated.Mouse embryo derived fibroblasts (NIH-3T3) were seeded on Si-containing calcium phosphate thin films.After 48 hours of cell seeding, fluorescence image of cells and the number of attached cells were observed using a microscope. As a result,the number of attached cells on the Si- containing calcium phosphate thin films increased with increasing Si content,and reached a maximum of 46 (cells/frame) on the film with 30% of SiO2 /SiO2 +HA target.Then the number of attached cells decreased to 24 (cells/frame) with the film with 100% of SiO2 target.


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