Elucidation of effect of serum with high TG concentration on lipase concentration measured using Cygnus Auto LIP
-
- KIYOSHIGE Atsushi
- Inspection Department, Kobe University Hospital
-
- WATANABE Yuki
- Inspection Department, Kobe University Hospital
-
- SATOU Itsuko
- Inspection Department, Kobe University Hospital
-
- NAKAMACHI Yuji
- Inspection Department, Kobe University Hospital
-
- IMANISHI Takamitsu
- Inspection Department, Kobe University Hospital
-
- SAEGUSA Jun
- Inspection Department, Kobe University Hospital
Bibliographic Information
- Other Title
-
- シグナスオートLIPに対して高TG血清が与える影響の解明
Search this article
Abstract
<p>[Introduction] A reagent for measuring lipase using the synthetic substrate 1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6-methyl-resorufin)-ester (DGGMR), which reacts specifically with lipase, has been developed. However, a high triglyceride (TG) concentration in serum was reported to cause a false negative error for lipase measured by “Cygnus Auto LIP.” Therefore, the purpose of this study was to clarify the effect of serum TG concentration on lipase concentration. [Method] We investigated the correlation between lipase concentration measured by Cygnus Auto LIP or Liquitech Lipase Color II and serum TG concentration. We studied the effect of each lipoprotein fraction on the change rate of lipase concentration. [Results] A high serum TG concentration caused a false negative error for lipase, and there was a significant negative correlation between them (r = −0.867, p ≤ 0.001). When serum TG concentrations exceeded 400 mg/dL, the false negative error for lipase reached 19%. A lipoprotein fraction analysis showed that VLDL had the strongest effect on the false negative error for lipase. [Discussion] Competition inhibition caused by lipoprotein particle size was considered to be a factor for the false negative error for lipase. Although it may have little impact on daily clinical practice, we need to pay attention to this error in serum samples with high TG concentrations, especially high VLDL or high chylomicron concentration.</p>
Journal
-
- Japanese Journal of Medical Technology
-
Japanese Journal of Medical Technology 69 (3), 360-365, 2020-07-25
Japanese Association of Medical Technologists
- Tweet
Details 詳細情報について
-
- CRID
- 1390848250131384960
-
- NII Article ID
- 130007880082
-
- ISSN
- 21885346
- 09158669
-
- Text Lang
- ja
-
- Data Source
-
- JaLC
- CiNii Articles
-
- Abstract License Flag
- Disallowed