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Ribosome rescue activity of an <i>Arabidopsis thaliana</i> ArfB homolog

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  • Nagao Michiaki
    Graduate School of Natural Science and Technology, Okayama University
  • Tsuchiya Fumina
    Graduate School of Integrated Science and Technology, Shizuoka University
  • Motohashi Reiko
    Graduate School of Integrated Science and Technology, Shizuoka University Research Institute of Green Science and Technology, Shizuoka University
  • Abo Tatsuhiko
    Graduate School of Natural Science and Technology, Okayama University

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  • Ribosome rescue activity of an Arabidopsis thaliana ArfB homolog

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Abstract

<p>A homolog of the bacterial ribosome rescue factor ArfB was identified in Arabidopsis thaliana. The factor, named AtArfB for Arabidopsis thaliana ArfB, showed ribosome rescue activity in both in vivo and in vitro assays based on the bacterial translation system. As has been shown for ArfB, the ribosome rescue activity of AtArfB was dependent on the GGQ motif, the crucial motif for the function of class I release factors and ArfB. The C-terminal region of AtArfB was also important for its function. The N-terminal region of AtArfB, which is absent in bacterial ArfB, functioned as a transit peptide for chloroplast targeting in tobacco cells. These results strongly suggest that AtArfB is a ribosome rescue factor that functions in chloroplasts.</p>

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