Cadmium-induced ABCB1 transporter expression in renal proximal tubular cells

DOI
  • MORI Chikage
    Laboratory of Pharmaceutical Health Sciences, School of Pharmacy, Aichi Gakuin University
  • LEE Jin-Yong
    Laboratory of Pharmaceutical Health Sciences, School of Pharmacy, Aichi Gakuin University
  • TOKUMOTO Maki
    Laboratory of Pharmaceutical Health Sciences, School of Pharmacy, Aichi Gakuin University
  • SATOH Masahiko
    Laboratory of Pharmaceutical Health Sciences, School of Pharmacy, Aichi Gakuin University

Bibliographic Information

Other Title
  • カドミウムによる腎近位尿細管でのABCB1トランスポーターの発現誘導

Abstract

<p>Our previous study demonstrated that cadmium (Cd) triggers renal toxicity through the disruption of transporting system. In this study, we investigated the effect of Cd on the expression level of ABCB1 (ATP binding cassette transporter subfamily B member 1) transporter in HK-2 human proximal tubular cells. Treatment of HK-2 cells with Cd up to 40μM for 6 h showed little cytotoxicity; however, Cd treatment for 24 h showed approximately 80% cytotoxicity. ABCB1 mRNA level was significantly increased by Cd treatment for 6 h in HK-2 cells. The protein level of P-glycoprotein (ABCB1 gene product) was also increased by Cd treatment for 6 and 12 h. These results suggested that the increase in not only gene expression of ABCB1 but also protein level of P-glycoprotein by Cd may induce some abnormalities in transport system in the kidney.</p>

Journal

Details 詳細情報について

  • CRID
    1390848647544943744
  • NII Article ID
    130007898290
  • DOI
    10.14869/toxpt.47.1.0_p-15s
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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