MEASURE: in vitro study for detection of residual undifferentiated pluripotent stem cells: (2) Highly Efficient Culture Assay

DOI
  • WATANABE Takeshi
    Drug Safety Research and Evaluation, Takeda Pharmaceutical Company Limited

Bibliographic Information

Other Title
  • MEASURE：多能性幹細胞検出のためのin vitro 試験 ：(2)培養増幅法

Abstract

<p>The highly efficient culture (HEC) assay is known as in vitro assay to detect residual undifferentiated pluripotent stem cells (PSCs) which possess tumorigenic potential. The MEASURE multisite experiments confirmed reported detection sensitivity (0.001%) under various conditions of hiPSC cell lines and culture medium/coating substrate. In addition, improved detection sensitivity (0.00002%) was achieved by utilizing magnetic activated cell sorting. These results indicate that HEC assay is highly sensitive and robust assay for detection of residual undifferentiated PSCs.</p>

Journal

Details 詳細情報について

  • CRID
    1390848647545136896
  • NII Article ID
    130007898578
  • DOI
    10.14869/toxpt.47.1.0_s22-4
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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