Dissimilatory Nitrate Reduction to Ammonium and Responsible Microbes in Japanese Rice Paddy Soil
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- Nojiri Yosuke
- Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- Kaneko Yuka
- Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- Azegami Yoichi
- Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- Shiratori Yutaka
- Niigata Agricultural Research Institute
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- Ohte Nobuhito
- Graduate School of Informatics, Kyoto University
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- Senoo Keishi
- Graduate School of Agricultural and Life Sciences, The University of Tokyo Collaborative Research Institute for Innovative Microbiology, The University of Tokyo
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- Otsuka Shigeto
- Graduate School of Agricultural and Life Sciences, The University of Tokyo Collaborative Research Institute for Innovative Microbiology, The University of Tokyo
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- Isobe Kazuo
- Graduate School of Agricultural and Life Sciences, The University of Tokyo
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抄録
<p>Nitrification–denitrification processes in the nitrogen cycle have been extensively examined in rice paddy soils. Nitrate is generally depleted in the reduced soil layer below the thin oxidized layer at the surface, and this may be attributed to high denitrification activity. In the present study, we investigated dissimilatory nitrate reduction to ammonium (DNRA), which competes with denitrification for nitrate, in order to challenge the conventional view of nitrogen cycling in paddy soils. We performed paddy soil microcosm experiments using 15N tracer analyses to assess DNRA and denitrification rates and conducted clone library analyses of transcripts of nitrite reductase genes (nrfA, nirS, and nirK) in order to identify the microbial populations carrying out these processes. The results obtained showed that DNRA occurred to a similar extent to denitrification and appeared to be enhanced by a nitrate limitation relative to organic carbon. We also demonstrated that different microbial taxa were responsible for these distinct processes. Based on these results and previous field observations, nitrate produced by nitrification within the surface oxidized layer may be reduced not only to gaseous N2 via denitrification, but also to NH4+ via DNRA, within the reduced layer. The present results also indicate that DNRA reduces N loss through denitrification and nitrate leaching and provides ammonium to rice roots in rice paddy fields.</p>
収録刊行物
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- Microbes and environments
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Microbes and environments 35 (4), n/a-, 2020
日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会
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詳細情報 詳細情報について
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- CRID
- 1390848647562056960
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- NII論文ID
- 130007923243
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- NII書誌ID
- AA11551577
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- ISSN
- 13474405
- 13426311
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- NDL書誌ID
- 031205579
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- PubMed
- 33028782
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可