3 次元ゲル包埋神経細胞コロニー間におけるネットワーク形成に及ぼす動的力学刺激の影響

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  • Effects of dynamics stimulation on forming neuronal network between 3-D gel embedded neurons colonies

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<p>In this study, we cultured two neighbor mouse neurons colonies in the 3-D gel embedded culture in differentiation process from iPS cells to neurons, and applied dynamic stimulation. We examined the effects of dynamic stimulation informing neuronal network between that colonies. To apply dynamic stimulation, we used a small piezoelectric vibration stage. Vibration conditions are set as the frequency:5Hz (12 hours stimulation-12 hours static and 14 hours stimulation- 10 hours static), the amplitude:60μm, and the culture period:7days, in horizontal direction. New evaluation methods were invented for connected neurites between two neurons colonies in the 3-D gel embedded culture. The result can be summarized as follows. Firstly, in vitro experiment system using two neurons colonies cultured by 3-D gel embedded culture was developed. Secondly, applying dynamic stimulation to two neurons colonies cultured in 3-D gel embedded culture, the effects of promoting differentiation and growth of whole neurons were observed. In particular, statistical significance was confirmed between the 14 hours stimulation-10 hours static and the static case. Thirdly, two new evaluation methods were invented. One method is focused on differentiation and growth about neurites, and the other is focused on neurites connection between two neuron colonies. Finally, possibility of increasing connected neurites network by applying dynamic stimulation was suggested with 12 hours of stimulation-12hours of static, and also 14 hours of stimulation-10 hours of static.</p>

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