Taxonomic studies of the species within the <i>Chloromonadinia</i> clade (Volvocales, Chlorophyceae), based on the comparative morphological analyses at the ultrastructural level and molecular data

DOI Web Site Web Site 27 References
  • Matsuzaki Ryo
    Faculty of Life and Environmental Sciences, University of Tsukuba

Bibliographic Information

Other Title
  • 微細構造レベルの比較形態解析と分子データに基づく緑藻クロロモナス系統群の種分類学的研究
  • ビサイ コウゾウ レベル ノ ヒカク ケイタイ カイセキ ト ブンシ データ ニ モトズク リョクソウ クロロモナス ケイトウグン ノ タネ ブンルイガクテキ ケンキュウ

Search this article

Abstract

<p>In order to promote the species taxonomy in microalgae, both detailed comparative morphological analysis and molecular phylogeny using cultured material are essential. I have focused on the Chloromonadinia clade (Volvocales, Chlorophyceae), which consists of morphologically and physiologically diverse species, and our research group has conducted taxonomic studies of this clade at the species level, based on comparative morphological analyses at the ultrastructural level and molecular data. Our previous reports of Chloromonas reticulata and its relatives showed that the species of which vegetative cells are morphologically similar to each other under light microscope, could be distinguished to the species level, based on ultrastructural differences in the pyrenoid or the eyespot within the chloroplast. In snow-inhabiting species belonging to the snow algae group within the Chloromonadinia clade, zygote morphologies are treated as the important taxonomic characteristics at the species level. However, induction of zygote formation (i.e. sexual reproduction) is difficult in experimental condition. Our comparative morphological analyses and molecular data using 19 strains of the snow algae group demonstrated that the strains could be subdivided into 12 species, based on differences in vegetative cell shape and chloroplast morphology, as well as in the number of zoospores within the parental cell wall and the presence or absence of cell aggregates in long-term cultures. In addition, we established the method that can obtain sequences of multiple DNA regions from field-collected zygotes of the snow algae group. The detailed molecular identification based on the sequence data unveiled that the part of Japanese field-collected materials morphologically assigned to the zygotes of C. brevispina and C. nivalis actually belong to the other species of the snow algae group. Besides, field-emission scanning electron microscopy for the zygotes of which the species were accurately identified by molecular data indicated that the ultrastructure on their zygote wall might represent a useful taxonomic trait at the species level.</p>

Journal

  • PLANT MORPHOLOGY

    PLANT MORPHOLOGY 32 (1), 91-97, 2020

    The Japanese Society of Plant Morphology

References(27)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top