Development of PCR-based DNA marker for detection of white carrot contamination caused by Y2 locus
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- Shibaya Taeko
- Fujii Seed Co. Ltd.
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- Kuroda Chika
- Fujii Seed Co. Ltd.
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- Nakayama Shinobu
- Kazusa DNA Research Institute
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- Minami Chiharu
- Kazusa DNA Research Institute
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- Obara Akiko
- Kazusa DNA Research Institute
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- Fujii Takayoshi
- Fujii Seed Co. Ltd.
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- Isobe Sachiko
- Kazusa DNA Research Institute
書誌事項
- タイトル別名
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- Development of PCR-based DNA marker for detection of white carrot contamination caused by <i>Y2</i> locus
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<p>In carrot (Daucus carota L.), the taproot colors orange, yellow and white are determined mostly by the Y, Y2, and Or loci. One of the most severe issues in carrot seed production is contamination by wild white carrot. To evaluate the contamination ratio, easily detectable DNA markers for white carrot are desired. To develop PCR-based DNA markers for the Y2 locus, we have re-sequenced two orange-colored carrot cultivars at our company (Fujii Seed, Japan), as well as six white- and one light-orange-colored carrots that contaminated our seed products. Within the candidate region previously reported for the Y2 locus, only one DNA marker, Y2_7, clearly distinguished white carrots from orange ones in the re-sequenced samples. The Y2_7 marker was further examined in 12 of the most popular hybrid orange cultivars in Japan, as well as ‘Nantes’ and ‘Chantenay Red Cored 2’. The Y2_7 marker showed that all of the orange cultivars examined had the orange allele except for ‘Beta-441’. False white was detected in the orange-colored ‘Beta-441’. The Y2_7 marker detected white root carrot contamination in an old open-pollinated Japanese cultivar, ‘Nakamura Senkou Futo’. This marker would be a useful tool in a carrot seed quality control for some cultivars.</p>
収録刊行物
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- Breeding Science
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Breeding Science 71 (2), 201-207, 2021
日本育種学会
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詳細情報 詳細情報について
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- CRID
- 1390851032061778816
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- NII論文ID
- 130008040488
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- NII書誌ID
- AA11317194
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- ISSN
- 13473735
- 13447610
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- NDL書誌ID
- 031481381
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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