Identification and Synthesis of DDI-6, a Quinolinol Analog Capable of Activating Both Caenorhabditis elegans and Mouse Spermatozoa

  • Karuo Yukiko
    Faculty of Pharmaceutical Sciences, Setsunan University
  • Shiraki Riona
    Department of Life Science, Faculty of Science and Engineering, Setsunan University
  • Yoshida Ayaka
    Department of Life Science, Faculty of Science and Engineering, Setsunan University
  • Tsunokawa Ryo
    Faculty of Pharmaceutical Sciences, Setsunan University
  • Nakahara-Yamada Mayuko
    Department of Life Science, Faculty of Science and Engineering, Setsunan University
  • Tarui Atsushi
    Faculty of Pharmaceutical Sciences, Setsunan University
  • Sato Kazuyuki
    Faculty of Pharmaceutical Sciences, Setsunan University
  • Kawai Kentaro
    Faculty of Pharmaceutical Sciences, Setsunan University
  • Omote Masaaki
    Faculty of Pharmaceutical Sciences, Setsunan University
  • Nishimura Hitoshi
    Department of Life Science, Faculty of Science and Engineering, Setsunan University

書誌事項

タイトル別名
  • Identification and Synthesis of DDI-6, a Quinolinol Analog Capable of Activating Both <i>Caenorhabditis elegans</i> and Mouse Spermatozoa

この論文をさがす

抄録

<p>Sperm activation is an essential process by which the male gametes become capable of fertilization. Because the process in Caenorhabditis elegans is readily reproducible in vitro, this organism serves as an excellent model to investigate it. C. elegans sperm activation in vivo occurs during spermiogenesis. Membranous organelles (MOs) contained within spermatids fuse with the plasma membrane, resulting in extracellular release of their contents and relocation of some proteins indispensable for fertilization from the MO membrane onto the sperm surface. Intriguingly, these cytological alternations are exhibited similarly in mouse spermatozoa during the acrosome reaction, which also represents a form of sperm activation, prompting us to hypothesize that C. elegans and mice share a common mechanism for sperm activation. To explore this, we first screened a chemical library to identify compounds that activate C. elegans spermatozoa. Because a quinolinol analog named DDI-6 seemed to be a candidate sperm activator, we synthesized it to use for further analyses. This involved direct dechlorination and hydrogenolysis of commercially available 5-chloro-8-quinolinol, both of which are key steps to yield 1,2,3,4-tetrahydro-8-quinolinol, and we subsequently introduced the sulfonamide group to the compound. When C. elegans spermatids were stimulated with solvent alone or the newly synthesized DDI-6, approx. 3% and approx. 28% of spermatids became MO-fused spermatozoa, respectively. Moreover, DDI-6 triggered the acrosome reaction in approx. 20% of mouse spermatozoa, while approx. 12% became acrosome-reacted after mock stimulation. Thus, DDI-6 serves as a moderately effective activator for both C. elegans and mouse spermatozoa.</p>

収録刊行物

被引用文献 (1)*注記

もっと見る

参考文献 (26)*注記

もっと見る

詳細情報 詳細情報について

問題の指摘

ページトップへ