Development of a method for quantification of serum dehydroepiandrosterone sulfate by stable isotope-dilution MALDI-MS

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  • Ogawa Shoujiro
    Faculty of Pharmaceutical Sciences, Tokyo University of Science Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University
  • Nakamura Masahiro
    Faculty of Pharmaceutical Sciences, Tokyo University of Science
  • Satoh Mamoru
    Division of Clinical Mass Spectrometry, Chiba University Hospital
  • Ishige Takayuki
    Division of Laboratory Medicine, Chiba University Hospital
  • Nomura Fumio
    Division of Clinical Genetics, Chiba University Hospital
  • Sugiura Yuki
    Department of Biochemistry, Keio University School of Medicine
  • Higashi Tatsuya
    Faculty of Pharmaceutical Sciences, Tokyo University of Science

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Abstract

<p>The quantification of dehydroepiandrosterone sulfate (DHEAS) in the human circulation might be of diagnostic help for several diseases, such as diabetes, depression, Alzheimer’s disease, osteoporosis and chronic heart failure. Matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) could be an effective approach to quantify the serum DHEAS due to its high specificity, handleability, rapid analysis and ruggedness. Nonetheless, to the best of our knowledge, no MALDI-MS assay has been reported for the quantification of DHEAS in biological samples. In this study, we developed and validated a method to quantify DHEAS in human serum by MALDI-MS using a stable isotope-dilution technique with 2H4-DHEAS as an internal standard. This method enabled the reproducible quantification of the serum DHEAS (intra- and inter-assay relative standard deviations, 9.3% or lower) with a small sample volume (20 μL) and effort-saving pretreatment (only deproteinization and crystallization with 9-aminoacridine). The measurable range was 0.250–5.00 μg/mL and the analysis time after the sample preparation was 3 min. The serum DHEAS concentrations measured by the newly-developed MALDI-MS method agreed well with those by liquid chromatography/electrospray ionization-MS/MS; this result demonstrated that the developed MALDI-MS method yielded reliable results.</p>

Journal

  • Medical Mass Spectrometry

    Medical Mass Spectrometry 5 (1), 22-27, 2021-06-25

    Japanese Society for Biomedical Mass Spectrometry

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