Simultaneous detection of monomers associated with resin-based dental composites using SPME and HPLC
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- KAKONYI Gabriella
- Groundwater Protection and Restoration Group, Department of Civil and Structural Engineering
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- MULLIGAN Steven
- School of Clinical Dentistry, Claremont Crescent, The University of Sheffield
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- FAIRBURN Andrew W.
- Groundwater Protection and Restoration Group, Department of Civil and Structural Engineering
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- MOHARAMZADEH Keyvan
- Hamdan Bin Mohammed College of Dental Medicine (HBMCDM), Mohammed Bin Rashid University of Medicine and Health Sciences (MBRU)
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- THORNTON Steven F.
- Groundwater Protection and Restoration Group, Department of Civil and Structural Engineering
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- WALKER Heather J.
- biOMICS Mass Spectrometry Facility, Department of Animal and Plant Sciences, Alfred Denny Building, Western Bank, The University of Sheffield
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- BURRELL Mike M.
- biOMICS Mass Spectrometry Facility, Department of Animal and Plant Sciences, Alfred Denny Building, Western Bank, The University of Sheffield
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- MARTIN Nicolas
- School of Clinical Dentistry, Claremont Crescent, The University of Sheffield
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Description
<p>As resin-based composites (RBC) replace dental amalgam for environmental reasons, there is a requirement to understand the environmental impact of this alternative dental restorative material. In this study we standardize the simultaneous detection of five monomeric components associated with RBCs using high performance liquid chromatography (HPLC) coupled with solid-phase microextraction (SPME). Factors affecting method performance (detection wavelength, calibration conditions, method sensitivity/accuracy/precision, extraction time/efficiency) are evaluated using standard solutions containing the mixture of TEGDMA, UDMA, Bis-GMA, BPA and HEMA. Detection sensitivity and analytical efficiency of the method is optimized for these compounds using 200 nm detection wavelength, PDMS/DVB fiber and extraction time of 90 min. Analytical accuracy of the HPLC is >95% for all monomers, with precision of 2.3–5.1%. Detection limits under the conditions described are 25 µg/L for HEMA, BPA, UDMA, Bis-GMA, and 100 µg/L for TEGDMA. The extraction time is governed by the largest molecular weight compounds.</p>
Journal
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- Dental Materials Journal
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Dental Materials Journal 40 (4), 1007-1013, 2021-07-25
The Japanese Society for Dental Materials and Devices
