Simple and Easy Synthesis of γ-Amido-dNTPs in Water and Their Polymerase Reaction Properties
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- Sakurada Takato
- Graduate School of Pharmaceutical Sciences, Kyushu University
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- Miyahara Ryo
- Graduate School of Pharmaceutical Sciences, Kyushu University
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- Kawazoe Ryoji
- Graduate School of Pharmaceutical Sciences, Kyushu University
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- Nagata Yusuke
- Graduate School of Pharmaceutical Sciences, Kyushu University
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- Kikukawa Yoshiya
- Graduate School of Pharmaceutical Sciences, Kyushu University
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- Sasaki Shigeki
- Graduate School of Pharmaceutical Sciences, Nagasaki International University
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- Taniguchi Yosuke
- Graduate School of Pharmaceutical Sciences, Kyushu University
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Description
<p>γ-Amido-modified 2′-deoxynucleoside triphosphates (dNTPs) and nucleoside triphosphates (NTPs) are becoming increasingly important as biological tools. We herein describe the simple and easy synthesis of γ-amido-dNTPs and -NTPs from commercially available corresponding dNTPs and NTPs in a one-pot reaction using water-soluble carbodiimide and ammonia solution. We examined the effects of synthesized γ-amido-dNTPs on the DNA polymerase reaction. The results obtained showed the incorporation of these derivatives into the DNA primer while maintaining nucleobase selectivity; however, their incorporation efficiency by DNA polymerase was lower than that of dNTP. This is the first study to demonstrate the successful synthesis of four sets of γ-amido-dNTPs and clarify their properties.</p>
Journal
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- Chemical and Pharmaceutical Bulletin
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Chemical and Pharmaceutical Bulletin 69 (11), 1061-1066, 2021-11-01
The Pharmaceutical Society of Japan
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Details 詳細情報について
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- CRID
- 1390852870557771904
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- NII Article ID
- 130008109895
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- NII Book ID
- AA00602100
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- ISSN
- 13475223
- 00092363
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- NDL BIB ID
- 031768619
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- PubMed
- 34719587
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL Search
- Crossref
- CiNii Articles
- KAKEN
- OpenAIRE
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- Abstract License Flag
- Disallowed