Leaf Bleaching in Rice : A New Disease in Vietnam Caused by Methylobacterium indicum, Its Genomic Characterization and the Development of a Suitable Detection Technique

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  • Lai Khoa
    United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology National Agro-Forestry-Fisheries Quality Assurance Department branch 4
  • Nguyen Ngoc Thai
    United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology
  • Yasuda Michiko
    Graduate School of Agriculture, Tokyo University of Agriculture and Technology
  • Dastogeer Khondoker M.G.
    Graduate School of Agriculture, Tokyo University of Agriculture and Technology Department of Plant Pathology, Bangladesh Agricultural University
  • Toyoda Atsushi
    Department of Genomics and Evolutionary Biology, National Institute of Genetics
  • Higashi Koichi
    Genome Evolution Laboratory, National Institute of Genetics
  • Kurokawa Ken
    Genome Evolution Laboratory, National Institute of Genetics
  • Nguyen Nga Thi Thu
    Department of Plant Protection, College of Agriculture and Applied Biology, CanTho University
  • Komatsu Ken
    United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology Graduate School of Agriculture, Tokyo University of Agriculture and Technology
  • Okazaki Shin
    United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology Graduate School of Agriculture, Tokyo University of Agriculture and Technology

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  • Leaf Bleaching in Rice: A New Disease in Vietnam Caused by <i>Methylobacterium indicum</i>, Its Genomic Characterization and the Development of a Suitable Detection Technique

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<p>A new disease in rice that is characterized by leaf bleaching was recently identified in some fields in the Mekong Delta region of Vietnam. The present study was the first to isolate and identify the pathogen of this disease. We confirmed that leaf bleaching symptoms were due to infection with Methylobacterium indicum bacteria using molecular biology approaches. A full-length genome analysis of pathogenic Methylobacterium strain VL1 revealed that it comprises a single chromosome and six plasmids, with a total size of 7.05‍ ‍Mbp and GC content of 70.5%. The genomic features of VL1 were similar to those of the non-pathogenic M. indicum strain SE2.11T; however, VL1 possessed additional unique genes, including those related to homoserine lactone biosynthesis. We established a loop-mediated isothermal amplification (LAMP) assay using the unique sequences of VL1 as target sequences for the rapid and simple detection of pathogenic M. indicum strains. Our initial evaluation demonstrated that the LAMP assay successfully distinguished between pathogenic and non-pathogenic strains infecting rice plants in a rapid and sensitive manner. The present results provide insights into the pathogenesis and development of control measures for novel rice diseases.</p>

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