COMPARISONS OF FISH DETECTION AMONG ENVIRONMENTAL DNA METABARCODING FROM THREE DIFFERENT GLASS-FIBER FILTER PORE SIZES AND CONVENTIONAL COLLECTION SURVEYS

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  • 異なる粒子保持径のガラスフィルターから得られた魚類の環境DNAメタバーコーディングと採集調査の比較

Abstract

<p> The objectives of this study are to (1) compare the results of fish environmental DNA(eDNA) metabarcoding from glass-fiber filters with different pore sizes and (2) compare these results with the results of fish collection surveys. We collected eDNA water samples and fishes in five rivers. We filtered the sampled water through the filters with three different pore sizes and analyzed the three types of filters individually for the eDNA metabarcoding. Our results showed that the filters with relatively small pore sizes (GF/F and GF/A) were effective in terms of the coverage of the collected fishes and the similarity to the collected fishes.</p>

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