Morphological analysis of renal organoids by transplantation of spheroids derived from the developing mouse kidney cells

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  • マウスの発生期腎臓細胞スフェロイドを移植した腎組織の形態解析
  • マウス ノ ハッセイキ ジンゾウ サイボウ スフェロイド オ イショク シタ ジンソシキ ノ ケイタイ カイセキ

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In recent years, organoids have been generated from various organs with the established iPS. However, the formation of capillary, which is the key renal organoid to hemofiltration, has not been successful yet. In this study, we investigated whether nephrons can be formed by introducing the microgravity environment combined with transplantation technology. Firstly, developing mouse kidney cells were cultured in a microgravity apparatus to form the fused spheroids. The formation of ureteric buds, nephron precursor and glomerular-like tissues were confirmed in the fused spheroids. Subsequently, the fused spheroids were transplanted under the mouse kidney capsule, which resulted in the formation of nephron-like tissue structure. Furthermore, the perfusion of fluorescent labeled albumin into the transplanted mouse showed albumin existing in the glomerular-like structure of the transplanted spheroid, and capillary structure revealed.

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