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- Mizuno Hajime
- School of Pharmaceutical Sciences, University of Shizuoka
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- Kudoh Shinobu
- Yokogawa Electric Corporation
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- Sugiyama Eiji
- School of Pharmaceutical Sciences, University of Shizuoka
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- Todoroki Kenichiro
- School of Pharmaceutical Sciences, University of Shizuoka
Bibliographic Information
- Other Title
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- Live Single-cell質量分析法による動物細胞内代謝物局在解析
- Live Single-cell シツリョウ ブンセキホウ ニ ヨル ドウブツ サイボウ ナイ タイシャブツキョクザイカイセキ
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Abstract
<p>We have developed “Live Single-cell Mass Spectrometry” technique for a direct analysis of endogenous compounds in a single living cell. The technique comprises a sampling from a living animal cell captured under video-microscopic observation, a nano-electrospray ionization of the sampled cell or cytosols and a high-resolution mass spectrometry. The technique with stable isotope-labeled reagents realizes quantitatively to trace the metabolic processes of endogenous compounds as well as xenobiotics in a live cell. In this paper, we demonstrate localization analysis of histidine metabolites in single living RBL-2H3 cells and to trace histidine metabolism using stable isotope labeling. This method has enabled us to analyze not only location of metabolic pathways but also metabolic processes of interest molecules, and it can be used in various organelle metabolomics in a single live cell. Currently, the technique is further improved and evolved to be a robotic cell manipulator with a confocal bio-imaging system so that a single cell or cytosol from a single live cell is automatically sampled based on a captured 3D bioimage.</p>
Journal
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- Regulation of Plant Growth & Development
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Regulation of Plant Growth & Development 55 (2), 92-99, 2020
The Japanese Society for Chemical Regulation of Plants
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Details 詳細情報について
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- CRID
- 1390853423037291520
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- NII Article ID
- 130008131328
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- NII Book ID
- AA11550064
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- ISSN
- 21896305
- 13465406
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- NDL BIB ID
- 031215709
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed