A brain atlas of a wild-type inbred strain of the medaka, Oryzias latipes

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  • A brain atlas of a wild-type inbred strain of the medaka, Oryzias latipes(<Special Issue>A Brain Atlas of Medaka)

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In response to the growing interest in the medaka (Oryzias latipes) as a model vertebrate for studies on neurobiology, we provide here an atlas of the adult brain of a wild-type inbred strain of the medaka. We used the HNI inbred strain, which has been used as a standard wild-type medaka in many laboratories. The adult brains were fixed and frontally cut into complete serial sections. The sections were stained with Nissl and Bodian methods. In the present atlas, external features of the brain, photographs of representative levels of the Nissl and Bodian sections, and drawings of these sections are presented. The brain structures were identified based on the recent comparative and hodological studies in various teleost species. In order to demarcate brain structures, hodological experiments using a carbocyanine dye (DiI) as a neuronal tracer have also been carried out. The atlas provides the basis for further developmental, neuroanatomical, neurophysiological, and behavioral investigations using the medaka.

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