An Efficient Protocol of Protocorm–Like Bodies Regeneration from Callus Cultures of Gastrodia elata Blume and the Further Associations with Mycorrhizal Fungi

DOI HANDLE 1 Citations Open Access
  • Yeh Chih-Hsin
    Institute of Tropical Agriculture, Faculty of Agriculture, Kyushu University | Taoyuan District Agricultural Research and Extension Station, Council of Agriculture
  • Liao Fang-Shin
    Institute of Tropical Agriculture, Faculty of Agriculture, Kyushu University | Taoyuan District Agricultural Research and Extension Station, Council of Agriculture
  • Huang Kuang-Liang
    Institute of Tropical Agriculture, Faculty of Agriculture, Kyushu University | Department of Horticultural Science, National Chiayi University
  • Miyajima Ikuo
    Institute of Tropical Agriculture, Faculty of Agriculture, Kyushu University
  • Lee Yung-I
    Institute of Tropical Agriculture, Faculty of Agriculture, Kyushu University | Biology Department, National Museum of Natural Science | Department of Life Sciences, National Chung Hsing University

Search this article

Description

In this study, we reported a reliable protocol of PLBs regeneration from embryogenic callus cultures of Gastrodia elata, a fully mycoheterotrophic orchid and an endangered herbal medicinal plant. The continuous immersion cultures with TDZ (0.5 and 1 mg · L^〈–1〉) and BA (10 mg · L^〈–1〉) improved PLBs regeneration frequency and rate. Histological studies indicated that starch grains were abundant within in the cytoplasm of embryogenic callus and developing PLBs. The formation of PLBs from cell aggregates in the periphery region of embryogenic callus was supposed to be the multicellular origin pathway. After inoculated with suitable Armillaria isolates, PLBs enlarged further and resulted in the formation of tubers. The results of phylogenetic analysis and inoculation experiments indicated that the asymbiotic PLBs could establish the appropriate association with Armillaria mellea complex, including A. mellea and A. gallica. Thus, this protocol of PLBs regeneration from callus cultures is beneficial for the multiplication of selected elites, and could be used as an experimental system for the molecular investigations of the mycoheterotrophic orchid – mycorrhizal fungi interactions.

Journal

Citations (1)*help

See more

Keywords

Details 詳細情報について

  • CRID
    1390853649614854144
  • NII Article ID
    120005981101
  • NII Book ID
    AA00247166
  • DOI
    10.5109/1798139
  • HANDLE
    2324/1798139
  • ISSN
    00236152
  • Text Lang
    en
  • Data Source
    • JaLC
    • IRDB
    • Crossref
    • CiNii Articles

Report a problem

Back to top