Preparation of Anti-fragrant Monoclonal Antibodies by the Rat Lymph Node Method and Their Characterization Using Enzyme-linked Immunosorbent Assay

DOI HANDLE 1 Citations Open Access
  • Ohashi Shintaro
    Laboratory of Food Analysis, Division of Food Biotechnology, Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University
  • Nagatomo Kazutaka
    Laboratory of Food Analysis, Division of Food Biotechnology, Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University
  • Miyamoto Koichiro
    Program of Food Science and Technology, Course of Applied Biological Science, Department of Bioresource and Bioenvironment, School of Agriculture, Kyushu University
  • Toko Kiyoshi
    Graduate School of Information Science and Electrical Engineering, Kyushu University
  • Miura Norio
    Art, Science and Technology Center for Cooperative Research, Kyushu University
  • Matsumoto Kiyoshi
    Laboratory of Food Analysis, Division of Food Biotechnology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University

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Abstract

In the food and beverage industries there is a great demand for a sensitive and easy detection system of fragrant compounds in order to ensure product quality control and to avoid contamination. Benzaldehyde (BZ) and furfural (FF) are typical fragrant compounds for peach?flavored beverages, and methyl anthranilate (MA) is a typical flavor of grape beverages. For sensitive detection of these flavors by immunoreaction, we prepared monoclonal antibodies against BZ, FF and MA by using the rat lymph node method. The antibody for BZ had high selectivity toward BZ compared with other fragrant compounds and high affinity with an affinity constant of 1.9×106M?1. The limit of detection (LOD) of BZ by indirect competitive ELISA (ic?ELISA) was 0.6 ng mL?1. For FF, anti?FF mAb had high selectivity toward FF and high affinity constant of 2.5×104 M?1. The LOD of FF by ic?ELISA was 100 ng mL?1. For MA, anti?MA mAb had high selectivity toward MA and high affinity constant of 1.3×105M?1. The LOD of MA by ic?ELISA was 200 ng mL?1. These monoclonal antibodies were suitable for detection of very small amounts of fragrant compounds in rinsed water of beverage industries.

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Details 詳細情報について

  • CRID
    1390853649614866048
  • NII Article ID
    120002223682
  • NII Book ID
    AA00247166
  • DOI
    10.5109/17808
  • HANDLE
    2324/17808
  • ISSN
    00236152
  • Text Lang
    en
  • Data Source
    • JaLC
    • IRDB
    • Crossref
    • CiNii Articles

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