In Vivo Metabolism of 2,2',3,4,4',5'-Hexachlorobiphenyl (CB138) in Guinea Pigs
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- Ohta Chiho
- Faculty of Nutritional Sciences, Nakamura Gakuen University
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- Haraguchi Koichi
- Daiichi College of Pharmaceutical Sciences
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- Kato Yoshihisa
- Kagawa School of Pharmaceutical Sciences, Tokushima Bunri University
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- Endo Tetsuya
- Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido
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- Koga. Nobuyuki
- Faculty of Nutritional Sciences, Nakamura Gakuen University
Bibliographic Information
- Other Title
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- 2,2',3,4,4',5'-六塩素化ビフェニル(CB138)のモルモットにおけるin vivo代謝
- 2 2 3 4 4 5 ロク エンソカ ビフェニル CB138 ノ モルモット ニ オケル in vivo タイシャ
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Abstract
Our preceding studies reported using animal liver microsomes that 2, 2', 3, 4, 4', 5'-hexachlorobiphenyl (hexaCB) (CB138), a worldwide and persistent organohalogen pollutant, was metabolized to two major hydroxy (OH)-metabolites, 3'-OH-CB138 (M-3) and 2'-OH-2,3,3',4,4', 5'-hexaCB (M-4), and two dechlorinated OH-metabolites (M-1 and M-2) in guinea pigs at much faster rate than in rats and hamsters. In this study, the distribution of four CB138 metabolites to the serum and liver 4 days after exposure and their fecal excretion were studied in guinea pigs administered with CB138 intraperitoneally. 3'-OH-CB138 (M-3) was a major metabolite in the liver, serum and feces. M-1 was observed as a minor metabolite in guinea pig feces. In contrast, trace amount of M-2 was present in guinea pig serum. However, 2'-OH-2,3,4,3',4',5'-hexaCB (M-4) which was a major metabolite in the in vitro system using guinea pig liver microsomes was not found in all tissues and feces tested in this study. On the other hand, the exact chemical structures of M-1 and M-2 were determined to be 6'-OH-2, 3, 3',4,4'-pentaCB and 4'-OH-2, 2',3,4,5'-pentaCB, respectively, by comparison of the retention time and mass fragmentation of the synthetic authentic amples in GC-MS. From these results, it is suggested that the metabolism of CB138 in guinea pigs may proceed by three pathways, a direct hydroxylation at 3'-position, and also the formation of 2',3'- or 3', 4'-epoxide and subsequent dechlorination and that three metabolites show the different mode of distribution and excretion.
Journal
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- 福岡醫學雜誌
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福岡醫學雜誌 102 (4), 167-174, 2011-04-25
Fukuoka Medical Association
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Keywords
Details 詳細情報について
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- CRID
- 1390853649693901440
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- NII Article ID
- 40018966705
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- NII Book ID
- AN00215478
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- DOI
- 10.15017/19739
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- HANDLE
- 2324/19739
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- NDL BIB ID
- 11215130
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- ISSN
- 0016254X
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- PubMed
- 21706897
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- Text Lang
- ja
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- Data Source
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- JaLC
- IRDB
- NDL
- PubMed
- CiNii Articles
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- Abstract License Flag
- Allowed