AN AMINO ACID SUBSTITUTION OF THE COAT PROTEIN OF PLUM POX VIRUS ALTERS ITS MOBILITY ON SDS-PAGE

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  • ウメ綸紋ウイルスの外被タンパク質のアミノ酸の違いによるウイルスの性状に関する研究

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Description

Plum pox virus (genus Potyvirus, family Potyviridae, PPV) is one of the most devastating plant viruses in the world. In 2009, PPV was first detected in Japanese apricot trees in Tokyo. We obtained four sample trees, Ak19-8, Ak19-9, Ak19-11, and Ak19-14, from a single field in Akiruno city, Tokyo. The symptoms of these trees were almost the same, however, by a Western-blot analysis, viral coat protein (CP) of Ak19-8 is apparently smaller than those of other three trees. We analyzed the sequences of their genomic RNAs and compared the deduced amino acid sequences of the CPs. We found difference in 66th amino acids, which was glycine in Ak19-8, while they were glutamic acid in Ak19-9, 11 and 14. This position is known to affect the manner of the modification of CP. It is also reported that CP was modified by O-linked N-acetylglucosamine (O-GlcNAc), which was suggested to work for host fitness. We separated the CPs of purified Ak19-8 and Ak19-14 particles by SDS-PAGE, and the CP bands were subjected to the MALDI-TOF MS analysis. The CP of Ak19-14 has two peaks correspond to the peptide of 40-93 amino acids carrying two and five O-GlcNAc, suggesting that the CP of the Japanese PPV are also modified by O-GlcNAc, and the manner of the modification could be different according to the 66th amino acid.

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Details 詳細情報について

  • CRID
    1390853649760907392
  • NII Article ID
    120005616103
  • NII Book ID
    AA12677220
  • DOI
    10.15002/00011444
  • HANDLE
    10114/10759
  • ISSN
    21879923
  • Text Lang
    ja
  • Article Type
    departmental bulletin paper
  • Data Source
    • JaLC
    • IRDB
    • CiNii Articles
  • Abstract License Flag
    Allowed

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