書誌事項
- タイトル別名
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- Optimal Bacterial DNA Isolation Method Using Bead-Beating Technique.
- サイキン DNA ブンリ ニ オケル ビーズホウ ノ オウヨウ
この論文をさがす
説明
Extraction of nucleic acids from Gram-positive bacteria is normally hampered by a thick and resistant cell wall. This paper presents procedures based on mechanical cell breakage to extract DNA from Staphylococcus aureus. The proposed system for DNA extraction involves bead-beating treatment and the GES method. These two steps allow a consistent extraction from the bacterium resistant to the GES method alone. Yield and quality of DNA obtained with the proposed method were higher than those obtained with the GES method alone. This protocol can be extended to clinical specimens.
収録刊行物
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- 九州大学医学部保健学科紀要
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九州大学医学部保健学科紀要 3 33-37, 2004-02
九州大学医学部保健学科
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詳細情報 詳細情報について
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- CRID
- 1390853649766429952
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- NII論文ID
- 110004683494
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- NII書誌ID
- AA11827066
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- ISSN
- 13482319
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- DOI
- 10.15017/3201
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- HANDLE
- 2324/3201
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- NDL書誌ID
- 6858629
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- IRDB
- NDL
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用可