Optimal Bacterial DNA Isolation Method Using Bead-Beating Technique.

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  • Fujimoto Shuji
    九州大学医学部保健学科 検査技術科学専攻
  • akagami Yoshiko
    九州大学医学部保健学科 検査技術科学専攻
  • Kojima Fumiko
    大阪大学大学院医学系研究科 医療技術科学 生体情報科学専攻 | 九州大学医学部保健学科 検査技術科学専攻

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  • 細菌DNA分離におけるビーズ法の応用
  • サイキン DNA ブンリ ニ オケル ビーズホウ ノ オウヨウ

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Abstract

Extraction of nucleic acids from Gram-positive bacteria is normally hampered by a thick and resistant cell wall. This paper presents procedures based on mechanical cell breakage to extract DNA from Staphylococcus aureus. The proposed system for DNA extraction involves bead-beating treatment and the GES method. These two steps allow a consistent extraction from the bacterium resistant to the GES method alone. Yield and quality of DNA obtained with the proposed method were higher than those obtained with the GES method alone. This protocol can be extended to clinical specimens.

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