Design of a Semi-confocal Fluorescence Microscope for Observing Excitation Spectrum of Soluble Molecules Adsorbed at the Air/water Interface

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  • Yang Haiya
    Interdisciplinary Graduate School of Engineering Sciences, Kyushu University
  • 原田 明
    九州大学大学院総合理工学研究院エネルギー物質科学部門 : 教授

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A semi-confocal fluorescence microscope is designed and constructed aiming to observe fluorescence excitation spectrum of soluble molecules adsorbed at the air/water interface. With a conventional confocal fluorescence microscope, the target molecules of pyrene arises some difficulties: excitation wavelength in deep ultraviolet forces low transmission performance of microscopic objectives and low wavelength-tunable performance of an excitation laser. A total internal reflection illumination with a light-guide coupled monochromated-xenon lamp is combined with the confocal fluorescence microscope. Theoretical feasibility and performance of the microscope are discussed with preliminary results obtained.

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  • Evergreen

    Evergreen 2 (2), 1-4, 2015-09

    九州大学グリーンテクノロジー研究教育センター

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