Fertility of Frozen Fowl Semen Stored for Long Term (9 Years)

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  • 長期間(9年)凍結保存した鶏精液の受精結果について
  • 長期間(9年)凍結保存した鶏精液の受精結果について〔英文〕
  • チョウキカン 9ネン トウケツ ホゾンシタ ニワトリ セイエキ ノ ジュセイ

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Abstract

Fowl semen diluted with 5% glucose egg-yolk solution which contained 7% glycerol in its final concentration and quickly frozen by liquid nitrogen was stored at -196°C for 9 years. The motility, abnormality and fertility of the spermatozoa in thawed semen were studied and showed the following results. 1. The percentage of motile spermatozoa in thawed semen stored for 9 years was 75% on the average. The spermatozoa motility was slightly low as compared with that of the previous ones which had been stored for 1/4, 2 and 5 years. 2. The percentage of abnormal spermatozoa in thawed semen stored for 9 years was 8.3% on the average. This spermatozoa abnormality is rather low as compared with that of our previous works and did not seem to be adversely affected by the long storage time. 3. The fertility of the thawed semen was 46.8% in the first week and 14.3% in the second week. The first week fertility decreased in some degree as compared with the previous three results and the second week fertility decreased more than that of 2 and 5 years storage with the exception of 1/4 year.

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