Analysis of amino acids by Orbitrap mass spectrometry.

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  • オービトラップ質量分析計を用いたアミノ酸解析の可能性
  • オービトラップ シツリョウ ブンセキケイ オ モチイタ アミノサン カイセキ ノ カノウセイ

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Abstract

Combination of a capillary electrophoresis interface ZipChipTMand Orbitrap Fusion mass spectrometer (Fusion MS) is applicable to amino acid analysis even from tiny amounts of biological samples, in the similar sensitivity to the conventional method using a gas chromatograph-mass spectrometer (GC-MS). Moreover, Fusion MS analysis can achieve simple preparation of samples and rapid detection of amino acids, which allow us to access cellular free amino acids with almost no preparation. Fusion MS analysis indeed potentially resolve the technical issues associated with the conventional GC-MS analysis, such as (1) conversion of asparagine and glutamine to asparaginate and glutamate, respectively, in protein hydrolysis and (2) low recovery of proline in sample preparation and no detection of arginine, cysteine, histidine, and tryptophan in GC-MS analysis. Here, we introduce sample preparation procedure for the analysis of free and total amino acids in bacterial cells and analytical conditions for the amino acid measurement in the Fusion MS method.

Journal

  • 低温科学

    低温科学 79 37-42, 2021-03-20

    低温科学第79巻編集委員会

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