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Acetylcholine Detecting Capacity of Leech Muscle as influenced by Potassium
Bibliographic Information
- Other Title
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- ヒル筋肉收縮によるアセチルコリン檢出に際しての共存加里の問題
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Description
When acetylcholine induces contraction in the eserinized smooth muscle of leech, the contraction is found to be modified characteristically by potassium ions existing in the solution containing the acetylcholine. The results obtained may be summarized as follows. The contraction due to acetylcholine is diminished by an addition of potassium salts to the solution (M/10 Frog's Ringer solution) in which the acetylcholine is dissolved, and so the contraction is established most efficiently in the acetylcholine solution freed from potassium ions. No such effect can be detected with calcium ions. It follows from these, that when the quantity of acetylcholine contained in a tissue extract is to be determined with the eserinized leech muscle, the potassium ions contained in the tissue extract must be taken into consideration, since cytoplasm in general is believed to be rich in potassium ions. The amount of acetylcholine in a solution to be tested is estimated usually by comparing the contraction of the leech muscle induced by the solution with those due to the standard solutions whose acetylcholine concentration is known, but, for this purpose, the concentration of potassium ions in the standard solutions must be identical with that of the solution in question. Hence, the amount of acetylcholine contained in a tissue extract whose potassium concentration is not known, can not be determined satisfactorily with the well known reaction of the leech muscle.
Journal
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- 動物学雑誌
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動物学雑誌 52 (1), 1-4, 1940-01-15
東京動物學會
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Details 詳細情報について
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- CRID
- 1390853651543575680
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- NII Article ID
- 110003333505
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- NII Book ID
- AN00166645
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- ISSN
- 00445118
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL Digital Collections (NII-ELS)
- CiNii Articles