アルギニンの単回摂取がラット骨格筋におけるmTORC1活性化に及ぼす影響

DOI Web Site Web Site 参考文献38件
  • 神崎 圭太
    川崎医療福祉大学医療技術学部臨床栄養学科
  • 山﨑 幸
    川崎医療福祉大学医療技術学部臨床栄養学科
  • 和田 正信
    広島大学大学院人間社会科学研究科

書誌事項

タイトル別名
  • Effects of single ingestion of arginine on mTORC1 activation in rat fast- and slow-twitch muscles
  • アルギニン ノ タンカイ セッシュ ガ ラット コッカクキン ニ オケル mTORC1 カッセイカ ニ オヨボス エイキョウ

この論文をさがす

抄録

<p>This study aimed to examine whether a single ingestion of arginine activates the mammalian target of rapamycin complex 1 (mTORC1) in rat fast- and slow-twitch skeletal muscles. In the first experiment, the rats were orally administered arginine (3 or 10 mmol/kg body weight) in water. The plantaris, gastrocnemius, and soleus muscles were excised 1 h after the administration. Immunoblot analysis showed that the administration with a higher dose (10 mmol/kg) resulted in increased phosphorylation of ribosomal S6 kinase (S6K) and ribosomal protein S6 only in the soleus muscles. The amounts of cellular arginine sensor for mTORC1 subunit 1 (CASTOR1) expressed were similar in these three muscles. In the second experiment, the plantaris and soleus muscles were excised 1 h after the administration of 10 mmol/kg of arginine. The binding of CASTOR1 to the GATOR2 complex was not detected in either muscle in co-immunoprecipitation and immunoblot analyses, irrespective of arginine administration. In the third experiment, a role of nitric oxide (NO) was elucidated. Treatment with an inhibitor of NO synthase blocked the arginine-induced increase in S6K phosphorylation. These results indicate that a single ingestion of arginine is capable of activating mTORC1 only in slow-twitch muscles and suggest that the activation may be mediated via NO, but not via the CASTOR1-GATOR2 complex pathway.</p>

収録刊行物

  • 体力科学

    体力科学 71 (2), 213-225, 2022-04-01

    一般社団法人日本体力医学会

参考文献 (38)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ