<i>Hericium erinaceum</i> (Yamabushitake) extract inhibits biofilm formation and degrades mature biofilm of the periodontopathogenic bacterium <i>Eikenella corrodens</i>

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  • IIDA Ryohei
    1) Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan 2) Department of Bioscience and Biotechnology, The United Graduate School of Agriculture, Tottori University, 4-101 Koyama-cho Minami, Tottori 680-8550, Japan
  • TANIGUCHI Shogo
    1) Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan
  • HAMAJI Momoko
    1) Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan
  • TAKAMUKU Miki
    1) Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan
  • ISHIDA Eriko
    1) Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan
  • SIDDIQA Ayesha
    1) Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan
  • AZAKAMI Hiroyuki
    1) Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan 3) Graduate School of Science and Technology for Innovation, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan 4) Research Center for Thermotolerant Microbial Resources, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan

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Other Title
  • <i>Hericium erinaceum</i>（ヤマブシタケ）抽出物が歯周病原性細菌<i>Eikenella corrodens</i>のバイオフィルム形成を阻害し，成熟したバイオフィルムを破壊する
  • Hericium erinaceum (Yamabushitake) extract inhibits biofilm formation and degrades mature biofilm of the periodontopathogenic bacterium Eikenella corrodens

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Abstract

Hericium erinaceum (Yamabushitake) is an edible mushroom that has been reported to display various pharmacological activities, including neuroprotective, anti-inflammatory, and antioxidant effects. In this study, we found that the water extract of H. erinaceum inhibited biofilm formation of the periodontopathogenic bacterium Eikenella corrodens; however, no inhibitory effect was observed with the methanol extract. Moreover, the biofilm inhibitory effect of H. erinaceum was observed in a dose-dependent manner and was not due to bactericidal activity against E. corrodens. H. erinaceum extract was fractionated by ammonium sulfate precipitation and biofilm inhibition was achieved with the 70% fraction. Heat treatment of the 70% fraction did not completely prevent biofilm inhibition, suggesting that partially heat-stable proteins are involved in the biofilm inhibition. Further fractionation of the 70% fraction by ultrafiltration indicated that the biofilm inhibitor may be a protein with a molecular weight of approximately 60 to 100 kDa. It is also suggested that H. erinaceum extract could degrade the biofilm once formed.

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