人工骨格筋収縮力測定デバイスの定量化に向けた取り組み

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  • Approaches to Quantification of Contractile Force Measurement Devices for Artificial Skeletal Muscle

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<p>Many human diseases are related to muscles, such as ALS and muscular dystrophy. The causes of these diseases are difficult to elucidate because of the interplay of numerous body systems in human, animal, and other species. We focused on an in vitro experimental strategy that uses organoids to mimic only muscle and eliminates the effects of the complex interrelationships between organs. Our group has successfully produced muscle organoids using C2C12 cells, a type of mouse cells, and collagen gels. We have developed a system that allows time-lapse observation of skeletal muscle under an inverted microscope without removing the skeletal muscle from the well plate. By applying electrical stimulation to the muscles, a contractile force is generated and the PDMS pillars tilts. The contractile force of the skeletal muscle is evaluated from the displacement of the PDMS pillars with an inverted microscope. However, In the conventional devices, the contraction force of the muscle was calculated using the Young's modulus of PDMS, and the measurement accuracy was limited. The PDMS pillars were calibrated to realize the more accurate quantitative evaluation with novel devices. Quantitative evaluation is expected to enhance the credibility of drug efficacy evaluation and electrical stimulation results. In the future, we hope to incorporate neural organoids as a new element in the device and apply it as a neuromuscular model device.</p>

収録刊行物

  • 年次大会

    年次大会 2021 (0), J302-04-, 2021

    一般社団法人 日本機械学会

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