Genetic diversity of Japanese isolates of pear chlorotic leaf spot-associated virus

TAKEYAMA S., SUZUKI R., KOHYAMA M., CHIAKI Y., TODA S., KUBOTA K.

doi:10.3186/jjphytopath.88.1

<p>Chlorotic leaf spot symptoms of Japanese and European pears, associated with infestation of the Japanese pear rust mite (Eriophyes chibaensis Kadono), have been noticed nationwide in Japan in the last decade. We previously reported detection of pear chlorotic leaf spot-associated virus (PCLSaV), a new emaravirus found in China, from pear orchards in Ibaraki, Niigata, Aichi, and Kumamoto prefectures, Japan. To further elucidate the regional distribution, relationship between the leaf chlorotic symptoms and PCLSaV infection, and the genetic diversity of PCLSaV isolates in Japan, we collected leaf samples of Japanese and European pear varieties with or without chlorotic spots from 13 prefectures from Honshu, Shikoku, and Kyushu regions in 2017, 2019, and 2020 and tested for RNA1–RNA5 of PCLSaV using reverse transcription-polymerase chain reaction. PCLSaV was detected in all the prefectures surveyed, and the chlorotic leaf spots were highly correlated with the presence of PCLSaV. Complete nucleotide sequences of open reading frames encoded by RNA1–RNA5 of the PCLSaV isolates were determined and phylogenetic and nucleotide diversity analyses were performed, which indicated that genetic diversity of the Japanese PCLSaV isolates was quite low compared to the three Chinese isolates reported previously. These results suggest that it appeared recently and rapidly spread in Japan.</p>

Genetic diversity of Japanese isolates of pear chlorotic leaf spot-associated virus

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