Newly Established Monoclonal Antibodies for Immunological Detection of H5N1 Influenza Virus
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- Ohnishi Kazuo
- Department of Immunology, National Institute of Infectious Diseases, Japan
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- Takahashi Yoshimasa
- Department of Immunology, National Institute of Infectious Diseases, Japan
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- Kono Naoko
- Influenza Virus Research Center, National Institute of Infectious Diseases, Japan
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- Nakajima Noriko
- Department of Pathology, National Institute of Infectious Diseases, Japan
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- Mizukoshi Fuminori
- Department of Immunology, National Institute of Infectious Diseases, Japan
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- Misawa Shuhei
- Tsuruga Institute of Biotechnology, Toyobo, Co., Ltd., Japan
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- Yamamoto Takuya
- Department of Immunology, National Institute of Infectious Diseases, Japan
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- Mitsuki Yu-ya
- Department of Immunology, National Institute of Infectious Diseases, Japan
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- Fu Shu-ichi
- Department of Immunology, National Institute of Infectious Diseases, Japan
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- Hirayama Nakami
- Department of Immunology, National Institute of Infectious Diseases, Japan
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- Ohshima Masamichi
- Department of Immunology, National Institute of Infectious Diseases, Japan
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- Ato Manabu
- Department of Immunology, National Institute of Infectious Diseases, Japan
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- Kageyama Tsutomu
- Influenza Virus Research Center, National Institute of Infectious Diseases, Japan
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- Odagiri Takato
- Influenza Virus Research Center, National Institute of Infectious Diseases, Japan
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- Tashiro Masato
- Influenza Virus Research Center, National Institute of Infectious Diseases, Japan
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- Kobayashi Kazuo
- Department of Immunology, National Institute of Infectious Diseases, Japan
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- Itamura Shigeyuki
- Influenza Virus Research Center, National Institute of Infectious Diseases, Japan
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- Tsunetsugu-Yokota Yasuko
- Department of Immunology, National Institute of Infectious Diseases, Japan
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<p>The H5N1 subtype of the highly pathogenic (HP) avian influenza virus has been recognized for its ability to cause serious pandemics among humans. In the present study, new monoclonal antibodies (mAbs) against viral proteins were established for the immunological detection of H5N1 influenza virus for research and diagnostic purposes. B-cell hybridomas were generated from mice that had been hyperimmunized with purified A/Vietnam/1194/2004 (NIBRG-14) virion that had been inactivated by UV-irradiation or formaldehyde. After screening over 4,000 hybridomas, eight H5N1-specific clones were selected. Six were specific for hemagglutinin (HA) and had in vitro neutralization activity. Of these, four were able to broadly detect all tested clades of the H5N1 strains. Five HA-specific mAbs detected denatured HA epitope(s) in Western blot analysis, and two detected HP influenza virus by immunofluorescence and immunohistochemistry. A highly sensitive antigen-capture sandwich ELISA system was established by combining mAbs with different specificities. In conclusion, these mAbs may be useful for rapid and specific diagnosis of H5N1 influenza. Therapeutically, they may have a role in antibody-based treatment of the disease.</p>
収録刊行物
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- Japanese Journal of Infectious Diseases
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Japanese Journal of Infectious Diseases 65 (1), 19-27, 2012-01-31
国立感染症研究所 Japanese Journal of Infectious Diseases 編集委員会
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詳細情報 詳細情報について
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- CRID
- 1390854717744819712
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- NII論文ID
- 40019139442
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- NII書誌ID
- AA1132885X
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- ISSN
- 18842836
- 13446304
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- NDL書誌ID
- 023392262
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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