Calcitonin Gene-related Peptide Inhibits Osteoclast Differentiation by Inducing the Negative Regulators MafB and Bcl6
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- Ishizuka Kyoko
- Department of Pharmacology, School of Dentistry, Aichi Gakuin University
書誌事項
- タイトル別名
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- Calcitonin Gene-related Peptide Inhibits Osteoclast Differentiation by Inducing the Negative Regulators <i>MafB</i> and <i>Bcl6</i>
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<p>Calcitonin gene-related peptide (CGRP), a neuropeptide derived from sensory nerves, suppresses osteoclast differentiation; however, the detailed mechanism is unknown. Therefore, in this study, we investigated the mechanism underlying the CGRP-mediated suppression of osteoclast differentiation using a model in which mouse monocyte/macrophage RAW264.7 cells were induced to differentiate into osteoclasts with recombinant mouse soluble receptor activator of nuclear factor-kappa B ligand (s-RANKL). RAW264.7 cells were cultured with s-RANKL in the presence or absence of CGRP for 24–72 h and then quantitative real-time PCR was performed. The mRNA expression of the positive regulators of osteoclast differentiation [i.e., nuclear factor of activated T-cells 1 (Nfatc1) and B lymphocyte-induced maturation protein-1 (Blimp1)], as well as that of the negative regulators of osteoclast differentiation [i.e., MAF bZIP transcription factor B (MafB), B-cell lymphoma 6 (Bcl6), and interferon regulatory factor-8 (Irf8)], was analyzed. In RAW264.7 cells treated with s-RANKL, CGRP significantly suppressed Nfatc1 and Blimp1 mRNA expression at 24 and 48 h. On the contrary, CGRP significantly increased the mRNA expression of MafB at 24 and 48 h of culture and of Bcl6 at 48 and 72 h of culture. However, CGRP did not affect Irf8 mRNA expression. In s-RANKL-untreated RAW264.7 cells, MafB mRNA expression significantly increased from 2 to 8 h, whereas Bcl6 mRNA expression significantly increased from 4 to 24 h of culture. These results suggest that CGRP inhibits osteoclast differentiation by increasing the mRNA expression of MafB and Bcl6, which are negative regulators of osteoclast differentiation.</p>
収録刊行物
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- Journal of Hard Tissue Biology
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Journal of Hard Tissue Biology 31 (2), 87-94, 2022
硬組織再生生物学会
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詳細情報 詳細情報について
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- CRID
- 1390854882572419456
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- NII書誌ID
- AA11074332
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- ISSN
- 1880828X
- 13417649
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- NDL書誌ID
- 032186964
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
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- 抄録ライセンスフラグ
- 使用不可