Isolating Venomous Constituents of <i>Paralepistopsis acromelalga</i>and Simultaneous Analysis of Its Compounds

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  • ドクササコに含有される有毒成分の精製および多成分同時分析法に関する研究
  • ドクササコ ニ ガンユウ サレル ユウドク セイブン ノ セイセイ オヨビ タセイブン ドウジ ブンセキホウ ニ カンスル ケンキュウ

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Abstract

<p>An isolation method for Acromelic acids A, B and Clitidine, which are venomous constituents of Paralepistopsis acromelalga was developed. Highly purified products were obtained from the mushroom extract using silica gel, ODS, ion-exchange column chromatography and preparative TLC. Using those results, we optimized the LC-MS/MS conditions. Finally we developed a method for simultaneous analysis. In recovery tests, the average recovery was 80.8–112.4%, repeatability was 1.4–3.8RSD%. The limits of quantification of the respective compounds were estimated as 0.25 μg/g. Based on the results, this method can reveal causes of food poisoning by Paralepistopsis acromelalga.</p>

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