<i>In situ</i> Imaging of Living Organisms by Raman and Near-infrared Spectroscopies — A look into the Brilliance of Life through Molecular Spectroscopies —

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  • ISHIGAKI Mika
    Institute of Agricultural and Life Sciences, Academic Assembly, Shimane University Raman Project Center for Medical and Biological Applications, Shimane University

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Other Title
  • ラマン分光法及び近赤外分光法による生体の<i>in situ</i>イメージング分析 — 分子分光分析を通して生命の輝きを視る —
  • ラマン分光法及び近赤外分光法による生体のin situイメージング分析 — 分子分光分析を通して生命の輝きを視る —
  • ラマン分光法及び近赤外分光法による生体のin situイメージング分析 : 分子分光分析を通して生命の輝きを視る
  • ラマン ブンコウホウ オヨビ キンセキガイ ブンコウホウ ニ ヨル セイタイ ノ in situ イメージング ブンセキ : ブンシ ブンコウ ブンセキ オ トオシテ セイメイ ノ カガヤキ オ ミル

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Abstract

<p>Raman spectroscopy can obtain spectral information strongly reflecting the molecular structure in a non-destructive manner, and near-infrared (NIR) spectroscopy can simultaneously analyze molecular composition, molecular concentration, and the interaction between the biomolecules such as proteins and water. By using both spectroscopies in a complementary manner, interdisciplinary research of analytical chemistry, physical chemistry, and biophysical chemistry have been achieved. In this review, three topics of my recent research are introduced. The first one is about non-staining in situ imaging of medaka fish eggs using NIR spectroscopy. The activation of egg development was captured by detecting the changes of the hydrogen bond network of water molecules, and optical interferences caused by Doppler effect made it possible to get blood flow images of fish embryos. With respect to the second topic, it was revealed that the mouse oocyte maturation and the embryonic quality were able to be assessed in situ by Raman spectroscopy. The concentration of lipids and phosphoric acids could be biomarkers for discriminating fertilization and viability competences of embryos, and the results presented a new possible evaluation method of embryonic quality to assisted reproductive technologies. As for the third topic, the distributions of lycopene aggregations within tomato fruits were visualized using resonance Raman imaging. J- and H-aggregates were distributed in layers under an exocarp, and the linear relationship between the frequencies of Raman band (ν1) and the electronic transition energy (S0→S2) shifted by forming aggregations was interpreted in terms of differences of effective C=C chain length. Since these spectroscopic imaging methods can obtain biomolecular information as they are, the methods are expected to be further applied to various fields such as medical treatment, pharmaceuticals, bioscience, and food engineering.</p>

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 71 (4.5), 221-233, 2022-04-05

    The Japan Society for Analytical Chemistry

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