Development of the High Sensitivity Measurement Method for Checking Cleanliness of Medical Instrumensts

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  • 医療器具に付着した蛋白質の高感度測定法について

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At present, the amide black 10B dyeing method is mainly used to make a final check for cleanliness of medical instruments after washing. But this method inbolber bisual inspertion for the final check and has problems in that it cannot be used to check an inner part of a tubular instrument that is impossible to view and that the check of contamination tends to be influenced by subjective judgment of an inspector. STo solve these problems, we attempted to quantify residual protein by using the protein dyeing agent Coomassie Brilliant Blue G-250. This agent permits quantifying residual contamination in cleaned medical instruments including the portions that cannot be viewed. Coomassie(R) Protein Assay Reagent Kit(PIERCE) was used as a protein dyeing agent. 0.2M sodium hydroxide liquid extrected protein at 50℃ for 30 minutes. Extraction liquid was used as a sample for fixed quantities. The coefficient of variation (C. V %) of this method ia as good as 2.3 to 3.3%. The pervention to this method by the antiseptic was not usually accepted by operating concentration. When the amount of protein which remains in scissors and suction tubes which were washed by the way washing processes, such asa a bliid coagulation prevention agent and wxistence of immersing washing, dirffer was measured by this method, the protein of high concentration which skipped the washing process was detected. These results showed that this method was very effective in the fixed quantity of the residual protein from the whole instrument.

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