Comprehensive detection of fusion transcripts in cell lines inferring pathogenic chimeras and chromosomal structural aberration in hematological malignancies

DOI
  • Kobayashi Tetsuko
    Department of Occupational Therapy, Faculty of Healcth Sciences，Kyorin University

Bibliographic Information

Other Title
  • 造血器腫瘍細胞株の融合転写産物の網羅的検出による染色体構造異常と病態形成に関わるキメラの推測

Abstract

<p>Comprehensive analyses of chimeras are crucial to understand molecular mechanisms of hematological malignancies. In this study, RNA-seq data of 1284 fusion transcripts of cell lines derived from hematological malignancies were analyzed using computer software. Chimeras representing chromosomal instability, that is, those with chromosomes of the same number whose distance between each breakpoint was under 1M base pairs (the detection limit of conventional karyotyping) and those formed by deletion or tandem repeat, accounted for three-quarters of all detected fusion transcripts, while balanced translocation/inversion accounted for 6.2%. There were four recurrent novel in-frame fusions (CLECL1-KLRB1, STEAP1B-RAPGEF5, TRDV2-TRAC, and YAF2-RYBP) and three recurrent long non-coding RNA involved in fusions (PFKFB3-LINC02649, LINC01934-ITGA4, and PVT1-CCDC26) , which were detected in numerous cell lines in multiple strains. In the future, detailed analyses using cell experiments and patient samples at each stage, and introduction of Hi-C method are needed. Ingenuity in handling RNA-seq data, analogous to genome structural analysis, may lead to an increase in the efficiency of chromosome analysis of hematological malignancies.</p>

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Keywords

Details 詳細情報について

  • CRID
    1390856539345866624
  • DOI
    10.11434/kyorinmed.53.97
  • ISSN
    1349886X
    03685829
  • Text Lang
    en
  • Data Source
    • JaLC
  • Abstract License Flag
    Disallowed

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