Evaluation of antimicrobial susceptibility testing of pathogen-positive blood cultures using MicroScan WalkAway

DOI Web Site
  • KITA Akiko
    Department of Clinical Laboratory, Mitsui Memorial Hospital
  • TAKEZAWA Riko
    Department of Clinical Laboratory, Mitsui Memorial Hospital
  • UBARA Hitomi
    Department of Clinical Laboratory, Mitsui Memorial Hospital
  • SUGA Toshimitsu
    Department of Clinical Laboratory, Mitsui Memorial Hospital
  • FUKUDA Yayoi
    Department of Clinical Laboratory, Mitsui Memorial Hospital
  • TSUCHIYA Tomoyuki
    Department of Clinical Laboratory, Mitsui Memorial Hospital
  • TAKEBATA Nanami
    Department of Clinical Laboratory, Mitsui Memorial Hospital
  • ONO Yuka
    Department of Clinical Laboratory, Mitsui Memorial Hospital

Bibliographic Information

Other Title
  • MicroScanWalkAwayを用いた血液培養陽性検体における薬剤感受性検査迅速法の検討
  • MicroScanWalkAway オ モチイタ ケツエキ バイヨウ ヨウセイケンタイ ニ オケル ヤクザイ カンジュセイ ケンサ ジンソクホウ ノ ケントウ

Search this article

Abstract

<p>At our hospital, the direct method of antimicrobial susceptibility testing of pathogen-positive blood cultures is performed by the disk diffusion method using the bottle culture medium. However, it is necessary to perform an indirect method to obtain the final result, and this takes several days. Therefore, to shorten the turn around time, we examined an indirect but rapid method (rapid method) for both the identification of causal microorganisms and antimicrobial susceptibility testing directly using the bottle culture medium and the autoanalyser MicroScan WalkAway 96plus (Beckman Coulter). We performed the rapid method using 353 blood culture samples (140 positive for cocci and 213 negative for bacilli) during the period at our hospital, and in parallel, we performed the direct disk diffusion method (disk method) and compared its results with those of the rapid method. About 98.0% were correctly identified by the rapid method. The categorical agreement was 79.2% for the disk method and 94.5% for the rapid method. The rapid method showed better results. The categorical agreement was ≥ 99% for Escherichia coli and ≥ 91% for Klebsiella pneumoniae. Although caution is required depending on the strain and antimicrobial agents, in the case of a single culture, the rapid method can be finished one day earlier than the current system, which is very beneficial in terms of both clinical and diagnostic aspects.</p>

Journal

Keywords

Details 詳細情報について

Report a problem

Back to top