Comparison of ESD and Conventional Methods in Single Cell Spray

DOI 機関リポジトリ (HANDLE)

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説明

Various inorganic ions are related to activity in a cell. Behavior of biological activity is traditionally observed by using optical microscope and/or MALDI. However, higher spatial resolution imaging technique is needed to observe organelles in a cell. In such cases, fixation in vacuum becomes quite big problem to maintain original structure in a cell. Rapid freezing seems one of the promising methods. But single cells cannot be treated as section. In this study, we devised a new rapid freezing method by combining electro-spray deposition and metal-touch freezing. In this method, individual cell is sprayed with a droplet emitted from the electro-spray tip, and each cells impact on the cooled metal surface, then frozen rapidly.

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詳細情報 詳細情報について

  • CRID
    1390857136564493952
  • DOI
    10.15002/00025845
  • HANDLE
    10114/00025845
  • ISSN
    02860201
  • 本文言語コード
    en
  • 資料種別
    departmental bulletin paper
  • データソース種別
    • JaLC
    • IRDB
  • 抄録ライセンスフラグ
    使用可

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