Microfluidic Device for Differentiation Induction of iPS Cells-derived Embryoid Bodies with Local Chemical Stimulation
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- Kusunoki Naoto
- Kagawa University
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- Konagaya Shuhei
- Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application, Kyoto University
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- Nishida Mitsunori
- TAZMO Co., Ltd.
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- Sato Shigehiro
- TAZMO Co., Ltd.
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- Takao Hidekuni
- Kagawa University
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- Shimokawa Fusao
- Kagawa University
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- Terao Kyohei
- Kagawa University
Bibliographic Information
- Other Title
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- iPS細胞胚様体の局所刺激を目指したマイクロデバイスの開発
Abstract
<p>This paper reports a microfluidic device that allows us to measure the effect of local differentiation stimulation on embryoid bodies for the purpose of elucidating the role of the cell-cell interaction of iPS cells in differentiation induction. We developed the local stimulation device having two chambers separated by thin polydimethyl siloxane (PDMS) membrane with through-holes of the diameter smaller than single cell. Embryoid bodies of iPS cells are formed on the membrane and seal the holes, separating the aqueous environments of 2 chambers without leakage. This configuration brings localized exposure to chemicals for differentiation induction at the area defined by the holes. In this paper, we demonstrated the function of local stimulation using fluorescent solution.</p>
Journal
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- IEEJ Transactions on Sensors and Micromachines
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IEEJ Transactions on Sensors and Micromachines 142 (12), 310-315, 2022-12-01
The Institute of Electrical Engineers of Japan
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Keywords
Details 詳細情報について
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- CRID
- 1390857226429016832
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- ISSN
- 13475525
- 13418939
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- Text Lang
- ja
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- Data Source
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- JaLC
- Crossref
- KAKEN
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- Abstract License Flag
- Disallowed