Mitigation of Paddy Field Soil Methane Emissions by Betaproteobacterium Azoarcus Inoculation of Rice Seeds
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- Sakoda Midori
- United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology
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- Tokida Takeshi
- Institute for Agro-Environmental Sciences, National Agriculture and Food Research Organization
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- Sakai Yoriko
- Institute for Agro-Environmental Sciences, National Agriculture and Food Research Organization
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- Senoo Keishi
- Graduate School of Agricultural and Life Sciences, The University of Tokyo Collaborative Research Institute for Innovative Microbiology, The University of Tokyo
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- Nishizawa Tomoyasu
- United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology Ibaraki University College of Agriculture
書誌事項
- タイトル別名
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- Mitigation of Paddy Field Soil Methane Emissions by Betaproteobacterium <i>Azoarcus</i> Inoculation of Rice Seeds
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説明
<p>Paddy fields are a major source of atmospheric methane, a greenhouse gas produced by methanogens and consumed by methanotrophs in flooded soil. The inoculation of rice seeds with the bacterium Azoarcus sp. KH32C alters the rice root-associated soil bacterial community composition. The present study investigated the effects of KH32C-inoculated rice cultivation on soil methanogens and methanotrophs involved in methane emissions from a rice paddy field. KH32C-inoculated and non-inoculated rice (cv. Nipponbare) were cultivated in a Japanese rice paddy with and without nitrogen fertilizer. Measurements of methane emissions and soil solution chemical properties revealed increases in methane flux over the waterlogged period with elevations in the concentrations of dissolved methane, dissolved organic carbon, and ferrous iron, which is an indicator of soil reduction levels. Reverse transcription quantitative PCR and amplicon sequencing were used to assess the transcription of the methyl-coenzyme M reductase gene (mcrA) from methanogens and the particulate methane monooxygenase gene (pmoA) from methanotrophs in paddy soil. The results obtained showed not only the transcript copy numbers, but also the compositions of mcrA and pmoA transcripts were related to methane flux. KH32C-inoculated rice cultivation recruited soil methanogens and methanotrophs that suppressed high methane synthesis, increased methane consumption, and decreased methane emissions by 23.5 and 17.2% under non-fertilized and nitrogen-fertilized conditions, respectively, while maintaining rice grain yield. The present study demonstrated the mitigation of paddy field methane emissions arising from the use of KH32C in rice cultivation due to its influence on the compositions of soil methanogen and methanotroph populations.</p>
収録刊行物
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- Ｍｉｃｒｏｂｅｓ ａｎｄ ｅｎｖｉｒｏｎｍｅｎｔｓ
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Ｍｉｃｒｏｂｅｓ ａｎｄ ｅｎｖｉｒｏｎｍｅｎｔｓ 37 (4), n/a-, 2022
日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境生物学会
