Genotypic analysis of the <i>FAS2-</i>F1279Y (3836T>A)<i> </i>polymorphism conferring high ethyl caprylate productivity in industrial sake yeast strains
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- Kuribayashi Takashi
- Niigata Agro-Food University
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- Sakurai Toshiki
- Niigata Agro-Food University
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- Hatakeyama Akira
- Yoshinogawa Co., Ltd.
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- Joh Toshio
- Faculty of Agriculture, Niigata University
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- Kaneoke Mitsuoki
- Niigata Prefectural Sake Research Institute
抄録
<p>In Saccharomyces cerevisiae, ethyl caprylate is produced by the esterification of caprylic acid, which is synthesized through the action of fatty acid synthase. A recent study reported a yeast mutant with a single nucleotide substitution in the alpha subunit of fatty acid synthase (FAS2) gene (F1279Y; 3836T>A) that produced large amounts of ethyl caprylate. Here, we designed two primer sets (P1/P2 and P3/P4) with mismatches that incorporate restriction sites for the enzymes NdeI and SspI, respectively and developed an easy and rapid polymerase chain reaction-restriction fragment length polymorphism assay to identify yeasts harboring the FAS2-F1279Y mutation associated with high ethyl caprylate productivity.</p>
収録刊行物
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- The Journal of General and Applied Microbiology
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The Journal of General and Applied Microbiology 68 (5), 248-252, 2022
公益財団法人 応用微生物学・分子細胞生物学研究奨励会