Effects of Intermittent Irradiation with Low-Level LED Light on Osteoblast-Like Cells Derived from Rat Bone Marrow

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  • Hattori Takehiro
    Department of Oral Pathology / Forensic Odontology, School of Dentistry, Aichi Gakuin University
  • Sugita Yoshihiko
    Department of Oral Pathology / Forensic Odontology, School of Dentistry, Aichi Gakuin University Research Institute of Advanced Oral Science, Graduate School of Dentistry, Aichi Gakuin University
  • Ogawa Akitaka
    Department of Fixed Prosthodontics and Oral Implantology, School of Dentistry, Aichi Gakuin University
  • Ito Yuki
    Department of Oral Pathology / Forensic Odontology, School of Dentistry, Aichi Gakuin University
  • Suzumura Toshikatsu
    Department of Oral Pathology / Forensic Odontology, School of Dentistry, Aichi Gakuin University
  • Isomura Madoka
    Department of Oral Pathology / Forensic Odontology, School of Dentistry, Aichi Gakuin University Department of Diagnostic Pathology, School of Medicine, Fujita Health University
  • Kawai Ryoko
    Department of Oral Pathology / Forensic Odontology, School of Dentistry, Aichi Gakuin University Research Institute of Advanced Oral Science, Graduate School of Dentistry, Aichi Gakuin University
  • Yoshida Waka
    Department of Oral Pathology / Forensic Odontology, School of Dentistry, Aichi Gakuin University Research Institute of Advanced Oral Science, Graduate School of Dentistry, Aichi Gakuin University
  • Kubo Katsutoshi
    Department of Oral Pathology / Forensic Odontology, School of Dentistry, Aichi Gakuin University Research Institute of Advanced Oral Science, Graduate School of Dentistry, Aichi Gakuin University
  • Horie Taku
    Department of Operative Dentistry, School of Dentistry, Aichi Gakuin University
  • Tomoda Shigetaka
    Department of Operative Dentistry, School of Dentistry, Aichi Gakuin University
  • Fujitani Morioki
    Department of Operative Dentistry, School of Dentistry, Aichi Gakuin University
  • Maeda Hatsuhiko
    Department of Oral Pathology / Forensic Odontology, School of Dentistry, Aichi Gakuin University Research Institute of Advanced Oral Science, Graduate School of Dentistry, Aichi Gakuin University

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<p>Recently, low level laser therapy (LLLT) has been reported to promote wound healing of fractures, tooth extraction sockets and bone-dental implants osseointegration. In this study, we investigated the effects of LLLT on cell proliferation, differentiation and calcification of rat bone marrow-derived osteoblast-like cells using blue LED light irradiation at a wavelength of 455 nm. The irradiation conditions were as follows; LED with a wavelength of 455 nm, 510 mW output power (measured value in the irradiated field: 100 mW/cm2), distance: 20.8 mm, and irradiation for 14 seconds every 1.5 hours at an energy of 5.6 joules/cm2 (up to 4.5 hours). The experimental groups were divided into 4 groups; control group, LED light-irradiation group, FGF-containing media group and LED light-irradiation and FGF-containing media group (n=3). The cell proliferative ability was determined using Cell Counting Kit-8 immediately after the first LED irradiation, after 1.5, 3.0, 4.5 and 12 hours by ELISA. Lab assay ALP was used to search for cell differentiation potential, and ALP activity levels in the samples were determined after 3 and 7 days. And the ALP staining was also performed after 7 days, and the ALP staining positive was determined by analyzing the percentage of stained area in each well. For the search of cellular calcification activity, Alizarin Red staining was used to determine its positivity rate by analyzing the stained area fraction of each well after 14 and 28 days. In all the searched results, both the cell proliferation and differentiation abilities were highest in the LED group. And the calcification ability was significantly higher in the LED, FGF and LED▪FGF groups than that in the control group. The results of this study suggested that LLLT with blue LED light irradiation was useful for bone tissue regeneration.</p>

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